Présentations dans le domaine du traitement de l'information, des systèmes embarqués et de l'automatique

Towards a quantitative description of subcellular phenotypes by Correlative Light and Electron Microscopy

Type : Séminaire
Orateur : Yannick Schwab (EMBL, Heidelberg, Germany)
Date : le mercredi 23 mai de 9h30 à 10h15
Lieu : Inria Rennes (bâtiment 12F du campus de Beaulieu), salle Métivier
Organisation : contacter laurent [dot] alberaatuniv-rennes1 [dot] fr (subject: S%C3%A9minaire%20%C3%A0%20venir) (Laurent Albera) afin qu'il prévienne de votre venue
Résumé : A variety of strategies are now available to correlate live cell or in vivo imaging to EM, but they very often suffer from tedious workflows. The Schwab team activities are centred on the development of new methods that increase the throughput of correlation on both cultured cells and multicellular specimens. By elaborating on the capacities of the focused ion beam - scanning electron microscope, automated correlation enables the acquisition of dozens of selected cells in a fully unattended fashion. The selection is performed by high-throughput screening at the light microscopic level which defines objective criteria to select the sub-populations of cells to be further analysed by electron microscopy. As a result, impact of various treatments, such as siRNA, on organelles morphology and distribution can be analysed at the ultrastructural level on multiple cells. On multicellular specimens, the major challenge is the navigation within the volume of the samples to reach to region of interest. Precise targeting is mandatory in order to make the correlation efficient. By using microscopic X-Ray computed tomography as a bridging modality, the 3D data obtained by in-vivo imaging, that reveal the targeted cells position, is registered to the topology of the resin embedded specimen. With this information, exposing the sub-volume of interest is fast and precise. This workflow opens to the transversal observation of dynamic events such a tumor-cell extravasation in a mouse model of brain metastasis.